This stain is NOT routinely used for sputum. You
want to use this test when you suspect Cyptococcus neoformans meningitis.
An example of this stain is below. This is the only yeast cell with a capsule. The capsule excludes the stain and a clear
zone is seen around each yeast cell.
Image property of J. Michael Miller, Ph.D., CDC, Atlanta, GA. Go to the following url for permissions and usage http://www.asm.org/Division/c/fungi.htm
I caught you!!! Learning something not specific to the case. What a wonderful concept. Learn on.