Acid-Fast Staining Instructions
1. Air dry and heat fix a thin film of microorganisms.
Allow the slide to cool.
2. Flood the slide with Carbolfuchsin.
Steam the slide with a Bunsen burner over the sink. Let the slide set for
5 minutes.
Rinse with water.
3. Flood slide with Acid Alcohol for 30
seconds. Rinse with water.
4. Counterstain by flooding the slide with Methylene
Blue for 30 seconds.
Rinse with water.
5. Dry the slide by putting it between the pages
of a book of Bibulous paper.
6. View organisms using the oil immersion objective
of your microscope.
©1996,1997 Neal R. Chamberlain, Ph.D. and Betty
Cox, M.A.. All rights reserved.